z-brain viewer matlab application  (MathWorks Inc)

Journal: Current biology : CB

Article Title: Hypothalamic Dopamine Neurons Control Sensorimotor Behavior by Modulating Brainstem Premotor Nuclei in Zebrafish

doi: 10.1016/j.cub.2020.09.002

Figure Lengend Snippet: (A) Experimental workflow. Fish were injected at the one-cell stage with a low concentration of the th2:gfp-aequorin plasmid to achieve sparse labeling. At 6 dpf, fish were fixed and stained for GFP and total ERK. Confocal stacks were transformed for registration to the z-brain atlas using the total ERK channel, and the same transformation was then applied to the GFP channel. Neurons were manually traced, and the anatomical locations of the soma and axonal termini were identified using the z-brain viewer in MATLAB [37]. Cyan boundaries mark annotated brain regions from the z-brain reference atlas.

Article Snippet: Neurons were manually traced, and the anatomical locations of the soma and axonal termini were identified using the z-brain viewer in MATLAB [ 37 ].

Techniques: Injection, Concentration Assay, Plasmid Preparation, Labeling, Staining, Transformation Assay